GTX17933

antibody from GeneTex

Targeting: FOS AP-1, c-fos

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX17933

Provider

GeneTex

Proper citation

GeneTex Cat#GTX17933, RRID:AB_422977

Product name

c-Fos (phospho Thr232) antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Extracts of SKBR3 cells were treated with 200ng/mL EGF for 10 minutes and resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 3% Milk-TBST buffer for one hour at room temperature, then incubated with the c-Fos [pT232] antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphothreonine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and signals were detected. The data show that only the phosphopeptide corresponding to c-Fos [pT232] blocks the signal, demonstrating the specificity of the antibody. While c-Fos [pT232] is phosphorylated in the basal state of SKBR3 (data not shown), EGF treatment induced an increase in the phosphorylation signal.