LS-C774971

antibody from LSBio

Targeting: FOS AP-1, c-fos

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C774971

Provider

LSBio

Product name

FOS / c-FOS Antibody (clone 5B10, SA) LS-C774971

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5B10

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot analysis of Human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Mouse Anti-c-fos Monoclonal Antibody, Clone 5B10. Lane 1: Molecular Weight Ladder (MW). Lane 2: Serum starved HeLa cell lysates. Lane 3: Serum starved HeLa cells treated with PMA (Phorbol 12-myristate 13-acetate). Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-c-fos Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 41 kDa. Other Band(s): 62 kDa. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.