MA5-36080

antibody from Invitrogen Antibodies

Targeting: FOS AP-1, c-fos

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-36080

Provider

Invitrogen Antibodies

Product name

c-Fos Recombinant Rabbit Monoclonal Antibody (14C10)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

14C10

Vial size

100 µL

Concentration

0.43 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Phospho-AKT1 (Thr450) in Hela cells using a Phospho-AKT1 (Thr450) monoclonal antibody (Product # MA5-36079) at a concentration of 2.25 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Phospho-AKT1 (Thr450) in Hela cells using a Phospho-AKT1 (Thr450) monoclonal antibody (Product # MA5-36079) at a concentration of 2.25 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of c-Fos in HepG2 cells using a c-Fos monoclonal antibody (Product # MA5-36080) at a dilution of 1:27. The cells were treated with 30mM sodium butyrate for 4 hours and counter stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the primary antibody overnight at 4°C. The secondary antibody was an Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of c-Fos in HepG2 cells using a c-Fos monoclonal antibody (Product # MA5-36080) at a dilution of 1:27. The cells were treated with 30mM sodium butyrate for 4 hours and counter stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the primary antibody overnight at 4°C. The secondary antibody was an Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of paraffin-embeded c-Fos in human adrenal gland tissue using a c-Fos monoclonal antibody (Product # MA5-36080) at a dilution of 1:81. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). The section was blocked with 10% normal goat serum for 30 min at room temperature. The primary antibody, in 1% BSA, was incubated at 4°C overnight. A biotinylated secondary antibody was used with an HRP conjugated SP system.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of c-Fos in Hela cells using a c-Fos monoclonal antibody (Product # MA5-36080) at a dilution of 1:50 is shown in red. The cells were fixed with 70% Ethylalcohol for 18 hours and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the primary antibody for 1 h at 4°C. A secondary FITC goat anti-rabbit IgG (H+L) antibody was used at 1/200 dilution for 1 hour at 4°C. The control, indicated in green, was used under the same conditions. An acquisition of >10,000 events was performed.