Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-36080 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Fos Recombinant Rabbit Monoclonal Antibody (14C10)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 14C10
- Vial size
- 100 µL
- Concentration
- 0.43 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Phospho-AKT1 (Thr450) in Hela cells using a Phospho-AKT1 (Thr450) monoclonal antibody (Product # MA5-36079) at a concentration of 2.25 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Phospho-AKT1 (Thr450) in Hela cells using a Phospho-AKT1 (Thr450) monoclonal antibody (Product # MA5-36079) at a concentration of 2.25 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of c-Fos in HepG2 cells using a c-Fos monoclonal antibody (Product # MA5-36080) at a dilution of 1:27. The cells were treated with 30mM sodium butyrate for 4 hours and counter stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the primary antibody overnight at 4°C. The secondary antibody was an Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of c-Fos in HepG2 cells using a c-Fos monoclonal antibody (Product # MA5-36080) at a dilution of 1:27. The cells were treated with 30mM sodium butyrate for 4 hours and counter stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the primary antibody overnight at 4°C. The secondary antibody was an Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded c-Fos in human adrenal gland tissue using a c-Fos monoclonal antibody (Product # MA5-36080) at a dilution of 1:81. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). The section was blocked with 10% normal goat serum for 30 min at room temperature. The primary antibody, in 1% BSA, was incubated at 4°C overnight. A biotinylated secondary antibody was used with an HRP conjugated SP system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of c-Fos in Hela cells using a c-Fos monoclonal antibody (Product # MA5-36080) at a dilution of 1:50 is shown in red. The cells were fixed with 70% Ethylalcohol for 18 hours and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the primary antibody for 1 h at 4°C. A secondary FITC goat anti-rabbit IgG (H+L) antibody was used at 1/200 dilution for 1 hour at 4°C. The control, indicated in green, was used under the same conditions. An acquisition of >10,000 events was performed.