Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- A00297-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-c-Fos/FOS Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for c-Fos/FOS detection. Tested with WB, FCM, Direct ELISA in Human.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Hops extract and xanthohumol ameliorate bone loss induced by iron overload via activating Akt/GSK3β/Nrf2 pathway.
Elevated c-fos expression is correlated with phenotypic switching of human vascular smooth muscle cells derived from lower limb venous varicosities.
Synergism between thioredoxin reductase inhibitor ethaselen and sodium selenite in inhibiting proliferation and inducing death of human non-small cell lung cancer cells.
Alterations in transcription and protein expressions of HCC-related genes in HepG2 cells caused by microcystin-LR.
Effect of monochromatic light on melatonin secretion and arylalkylamine N-acetyltransferase mRNA expression in the retina and pineal gland of broilers.
Genistein induces G2/M cell cycle arrest via stable activation of ERK1/2 pathway in MDA-MB-231 breast cancer cells.
Effects of isoliensinine on angiotensin II-induced proliferation of porcine coronary arterial smooth muscle cells.
Sun X, Xia T, Zhang S, Zhang J, Xu L, Han T, Xin H
Journal of bone and mineral metabolism 2022 May;40(3):375-388
Journal of bone and mineral metabolism 2022 May;40(3):375-388
Elevated c-fos expression is correlated with phenotypic switching of human vascular smooth muscle cells derived from lower limb venous varicosities.
Guo Z, Luo C, Zhu T, Li L, Zhang W
Journal of vascular surgery. Venous and lymphatic disorders 2021 Jan;9(1):242-251
Journal of vascular surgery. Venous and lymphatic disorders 2021 Jan;9(1):242-251
Synergism between thioredoxin reductase inhibitor ethaselen and sodium selenite in inhibiting proliferation and inducing death of human non-small cell lung cancer cells.
Zheng X, Xu W, Sun R, Yin H, Dong C, Zeng H
Chemico-biological interactions 2017 Sep 25;275:74-85
Chemico-biological interactions 2017 Sep 25;275:74-85
Alterations in transcription and protein expressions of HCC-related genes in HepG2 cells caused by microcystin-LR.
Li X, Zhang X, Xie W, Zhou C, Li Y, Zhang X
Toxicology in vitro : an international journal published in association with BIBRA 2017 Apr;40:115-123
Toxicology in vitro : an international journal published in association with BIBRA 2017 Apr;40:115-123
Effect of monochromatic light on melatonin secretion and arylalkylamine N-acetyltransferase mRNA expression in the retina and pineal gland of broilers.
Jin E, Jia L, Li J, Yang G, Wang Z, Cao J, Chen Y
Anatomical record (Hoboken, N.J. : 2007) 2011 Jul;294(7):1233-41
Anatomical record (Hoboken, N.J. : 2007) 2011 Jul;294(7):1233-41
Genistein induces G2/M cell cycle arrest via stable activation of ERK1/2 pathway in MDA-MB-231 breast cancer cells.
Li Z, Li J, Mo B, Hu C, Liu H, Qi H, Wang X, Xu J
Cell biology and toxicology 2008 Oct;24(5):401-9
Cell biology and toxicology 2008 Oct;24(5):401-9
Effects of isoliensinine on angiotensin II-induced proliferation of porcine coronary arterial smooth muscle cells.
Xiao JH, Zhang YL, Feng XL, Wang JL, Qian JQ
Journal of Asian natural products research 2006 Apr-May;8(3):209-16
Journal of Asian natural products research 2006 Apr-May;8(3):209-16
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of c-Fos/FOS using anti-c-Fos/FOS antibody (A00297-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Sh-sy5y whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Fos/FOS antigen affinity purified polyclonal antibody (Catalog # A00297-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Fos/FOS at approximately 50KD. The expected band size for c-Fos/FOS is at 50KD.
- Additional image