RQ5724

antibody from NSJ Bioreagents

Targeting: FOS AP-1, c-fos

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5724

Provider

NSJ Bioreagents

Product name

c-Fos Antibody / FOS

Antibody type

Polyclonal

Description

This highly specific c-Fos antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the c-Fos antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) Jurkat, 3) SH-SY5Y and 4) A549 cell lysate with c-Fos antibody. Expected molecular weight: ~40 kDa (unmodified), 53-68 kDa (phosphorylated).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human SiHa cells with c-Fos antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= c-Fos antibody.