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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- STJA0003594 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-c-FOS antibody [2H2] (STJA0003594)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-c-FOS is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C, and avoid repeated freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence of a section of rat hippocampus showing neurons labeled with c-FOS (cat. STJA0003594, 1:1000, red) counterstained with FOX3 (NeuN) (green) , and nuclear staining with DAPI. When both c-FOS and FOX3 are expressed the label appears orange.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti-c-FOS (cat. STJA0003594, 1:1000, green) while Anti-Vimentin (cat. STJA0003823, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunostaining of mouse brain labeling c-Fos protein in green (cat. STJA0003594, 1:1000) and DNA with DAPI.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunolabeling of a section of rat brain that specifically labels c-FOS protein (cat. STJA0003594, green, 1:1000). Frozen sections were fixed with 4%PFA for 24 hours. Before performing free floating immunohistochemistry, sections underwent antigen retrieval with sodium citrate (pH6. 0) for 30 minutes at 80˚C. Image kindly provided by Angela Gonzalez, Washington State University, Vancouver.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
