Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX101196 - Provider product page
- Provider
- GeneTex
- Product name
- c-Fos antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- c-Fos antibody detects FOS protein by Western blot analysis.A. 30 ?g MCF-7 whole cell lysate/extract (serum starvation)B. 30 ?g MCF-7 whole cell lysate/extract (500nM PMA treatment for 0.5hr)C. 30 ?g MCF-7 whole cell lysate/extract (500nM PMA treatment for 1hr)D. 30 ?g MCF-7 whole cell lysate/extract (500nM PMA treatment for 2hr)10 % SDS-PAGEc-Fos antibody (GTX101196) dilution: 1:5000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- c-Fos antibody detects c-Fos protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: c-Fos protein stained by c-Fos antibody (GTX101196) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Scale bar = 10 £gm.