NB300-275

antibody from Novus Biologicals

Targeting: EFNB1 CFNS, Elk-L, EPLG2, LERK2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

NB300-275

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-275, RRID:AB_10001250

Product name

Rabbit Polyclonal Ephrin-B1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specific for the ~46k EphrinB protein phosphorylated at Tyr317. The immunolabeling of the EphrinB band is blocked by treatment with lambda-phosphatase.

Reactivity

Mouse, Rat, Chicken/Avian

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Ephrin-B reverse signaling controls septation events at the embryonic midline through separate tyrosine phosphorylation-independent signaling avenues.

Dravis C, Henkemeyer M
Developmental biology 2011 Jul 1;355(1):138-51

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ephrin-B1 [p Tyr317] Antibody [NB300-275] - Rat testes lysate showing specific immunolabeling of the ~46k EphriNBphosphorylated at Tyr317 (control, lane 1). The phosphospecificity of this labeling is shown in lane 2 (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the Anti-Tyr317 EphrinB. The immunolabeling of the EphriNBis completely eliminated by treatment with lambda-phosphatase.