Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF473 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Ephrin-B1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Ephrin-B1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Ephrin-A3, rhEphrin-A4, recombinant mouse (rm) Ephrin-A1, rmEphrin-B2, rhEphrin-A5, rhEphrin-B3, rmEphrin-A2, rmEphrin-A4, and recombinant zebrafish Ephrin-B2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAA53231- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Functional Consequences of Synapse Remodeling Following Astrocyte-Specific Regulation of Ephrin-B1 in the Adult Hippocampus.
Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation.
Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
Tissue-Specific Effects of Reduced β-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model.
Cortical abnormalities and non-spatial learning deficits in a mouse model of CranioFrontoNasal syndrome.
Ephrin-B1 controls the columnar distribution of cortical pyramidal neurons by restricting their tangential migration.
Possible role of Efnb1 protein, a ligand of Eph receptor tyrosine kinases, in modulating blood pressure.
Ephrin-B reverse signaling promotes structural and functional synaptic maturation in vivo.
Binding of ras to phosphoinositide 3-kinase p110alpha is required for ras-driven tumorigenesis in mice.
Inhibition of inflammatory lymphangiogenesis by integrin alpha5 blockade.
The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
Intracellular and trans-synaptic regulation of glutamatergic synaptogenesis by EphB receptors.
EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
EphrinB1 is essential in T-cell-T-cell co-operation during T-cell activation.
Koeppen J, Nguyen AQ, Nikolakopoulou AM, Garcia M, Hanna S, Woodruff S, Figueroa Z, Obenaus A, Ethell IM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jun 20;38(25):5710-5726
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jun 20;38(25):5710-5726
Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation.
O'Neill AK, Kindberg AA, Niethamer TK, Larson AR, Ho HH, Greenberg ME, Bush JO
The Journal of cell biology 2016 Oct 24;215(2):217-229
The Journal of cell biology 2016 Oct 24;215(2):217-229
Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
Geng X, Cha B, Mahamud MR, Lim KC, Silasi-Mansat R, Uddin MKM, Miura N, Xia L, Simon AM, Engel JD, Chen H, Lupu F, Srinivasan RS
Developmental biology 2016 Jan 1;409(1):218-233
Developmental biology 2016 Jan 1;409(1):218-233
Tissue-Specific Effects of Reduced β-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
Feng Y, Sakamoto N, Wu R, Liu JY, Wiese A, Green ME, Green M, Akyol A, Roy BC, Zhai Y, Cho KR, Fearon ER
PLoS genetics 2015 Nov;11(11):e1005638
PLoS genetics 2015 Nov;11(11):e1005638
GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model.
Dong LD, Gao F, Wang XH, Miao Y, Wang SY, Wu Y, Li F, Wu J, Cheng XL, Sun XH, Yang XL, Wang Z
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Apr 1;35(13):5409-21
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Apr 1;35(13):5409-21
Cortical abnormalities and non-spatial learning deficits in a mouse model of CranioFrontoNasal syndrome.
Arvanitis DN, Behar A, Drougard A, Roullet P, Davy A
PloS one 2014;9(2):e88325
PloS one 2014;9(2):e88325
Ephrin-B1 controls the columnar distribution of cortical pyramidal neurons by restricting their tangential migration.
Dimidschstein J, Passante L, Dufour A, van den Ameele J, Tiberi L, Hrechdakian T, Adams R, Klein R, Lie DC, Jossin Y, Vanderhaeghen P
Neuron 2013 Sep 18;79(6):1123-35
Neuron 2013 Sep 18;79(6):1123-35
Possible role of Efnb1 protein, a ligand of Eph receptor tyrosine kinases, in modulating blood pressure.
Wu Z, Luo H, Thorin E, Tremblay J, Peng J, Lavoie JL, Wang Y, Qi S, Wu T, Wu J
The Journal of biological chemistry 2012 May 4;287(19):15557-69
The Journal of biological chemistry 2012 May 4;287(19):15557-69
Ephrin-B reverse signaling promotes structural and functional synaptic maturation in vivo.
Lim BK, Matsuda N, Poo MM
Nature neuroscience 2008 Feb;11(2):160-9
Nature neuroscience 2008 Feb;11(2):160-9
Binding of ras to phosphoinositide 3-kinase p110alpha is required for ras-driven tumorigenesis in mice.
Gupta S, Ramjaun AR, Haiko P, Wang Y, Warne PH, Nicke B, Nye E, Stamp G, Alitalo K, Downward J
Cell 2007 Jun 1;129(5):957-68
Cell 2007 Jun 1;129(5):957-68
Inhibition of inflammatory lymphangiogenesis by integrin alpha5 blockade.
Dietrich T, Onderka J, Bock F, Kruse FE, Vossmeyer D, Stragies R, Zahn G, Cursiefen C
The American journal of pathology 2007 Jul;171(1):361-72
The American journal of pathology 2007 Jul;171(1):361-72
The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
Yang NY, Pasquale EB, Owen LB, Ethell IM
The Journal of biological chemistry 2006 Oct 27;281(43):32574-86
The Journal of biological chemistry 2006 Oct 27;281(43):32574-86
Intracellular and trans-synaptic regulation of glutamatergic synaptogenesis by EphB receptors.
Kayser MS, McClelland AC, Hughes EG, Dalva MB
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Nov 22;26(47):12152-64
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Nov 22;26(47):12152-64
EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
Journal of cell science 2006 Feb 1;119(Pt 3):559-70
Journal of cell science 2006 Feb 1;119(Pt 3):559-70
EphrinB1 is essential in T-cell-T-cell co-operation during T-cell activation.
Yu G, Luo H, Wu Y, Wu J
The Journal of biological chemistry 2004 Dec 31;279(53):55531-9
The Journal of biological chemistry 2004 Dec 31;279(53):55531-9
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Ephrin-B1 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Ephrin-B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF473) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Ephrin-B1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Ephrin-B1 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Ephrin-B1 at approximately 65 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse Ephrin-B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF473) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Ephrin-B1 in Mouse Kidney. Ephrin-B1 was detected in immersion fixed frozen sections of mouse kidney using Goat Anti-Mouse Ephrin-B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF473) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
