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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-62772 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OTUD6A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: HRQELEKFQD DSSIESVVED LAKMNLENRP PRSSKAHRKR ERMESEERER QESIFQAEMS EHLAGFKREE E Highest antigen sequence identity to the following orthologs: Mouse - 53%, Rat - 51%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references OTUD6A promotes prostate tumorigenesis via deubiquitinating Brg1 and AR.
Fu X, Zhao J, Yu G, Zhang X, Sun J, Li L, Yin J, Niu Y, Ren S, Zhu Y, Xu B, Huang L
Communications biology 2022 Mar 1;5(1):182
Communications biology 2022 Mar 1;5(1):182
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OTUD6A in control (vector only transfected HEK293T lysate) and OTUD6A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a OTUD6A Polyclonal Antibody (Product # PA5-62772).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of OTUD6A in human testis using OTUD6A Polyclonal Antibody (Product # PA5-62772) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of OTUD6A in human endometrium using OTUD6A Polyclonal Antibody (Product # PA5-62772) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 OTUD6A oligonucleotides markedly suppress prostate tumorigenesis derived from Pten PC-/- mice and patient-derived xenograft (PDX) models. a, b Established Pten PC-/- male mice were injected with AAV-GFP or AAV-sh OTUD6A into VP tissues at 2-month-age. Three months later, VP part of prostates were imaged ( a ) and weighted ( b ) ( n = 5-6). The Pten fl/fl mice were used as the negative control. Data represented the mean +- SEM. c H&E-stained sections of representative VP tissues, scale bars: 100 mum. d IB analysis of PTEN and OTUD6A in different mouse prostates. e - g The PCa PDX model was constructed and the PDX tumor tissues were subcutaneously injected into the flank of 6-week-old NOD/SCID mice, and then AAV-GFP or AAV-sh OTUD6A were intratumorally administrated. The growth curves were shown in ( e ), and the mean tumor growth inhibition rate (TGI) of each monitoring point was shown in ( f ). The dissected tumors were collected and weighed ( g ) around 4 weeks later ( n = 6). Data represented the mean +- SEM. Statistical significance was determined by two-tailed unpaired Student's t -test, * P < 0.05, ** P < 0.01, *** P < 0.001. h Equal amounts of proteins from tumor tissues were evaluated for OTUD6A.
