PAB28120

antibody from Abnova Corporation

Targeting: C4orf19 FLJ11017

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28120

Provider

Abnova Corporation

Product name

C4orf19 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant C4orf19.

Antigen sequence

VPSPGYVNEVNSCKLDEDDTDKLKGKWSSEVLVQK
NDPQRQGSKKTESSSRTADPWEPCWPHQGPLPQGD
AGGEHHACGVNGIGPAATPQPTGNSSPT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with C4orf19 polyclonal antibody (Cat # PAB28120) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with C4orf19 polyclonal antibody (Cat # PAB28120) at 1-4 ug/mL dilution shows positivity in cytoplasm and cytoskeleton (intermediate filaments).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with C4orf19 polyclonal antibody (Cat # PAB28120) shows moderate membranous positivity in glandular cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)