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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- PA5-60368 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C4orf19 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VPSPGYVNEV NSCKLDEDDT DKLKGKWSSE VLVQKNDPQR QGSKKTESSS RTADPWEPCW PHQGPLPQGD AGGEHHACGV NGIGPAATPQ PTGNSSPT Highest antigen sequence identity to the following orthologs: Mouse - 47%, Rat - 44%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C4orf19 in control (vector only transfected HEK293T lysate) and C4orf19 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a C4orf19 Polyclonal Antibody (Product # PA5-60368).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of C4orf19 in human cell line RT4 shows positivity in cell junctions. Samples were probed using a C4orf19 Polyclonal Antibody (Product # PA5-60368).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of C4orf19 in human cell line RT4 using a C4orf19 Polyclonal Antibody (Product # PA5-60368) shows localization to microtubule organizing center and cell junctions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C4orf19 in human stomach and skin tissues using C4orf19 Polyclonal Antibody (Product # PA5-60368). Corresponding C4orf19 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C4orf19 in human stomach using C4orf19 Polyclonal Antibody (Product # PA5-60368) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of C4orf19 in human skin using C4orf19 Polyclonal Antibody (Product # PA5-60368) shows low expression as expected.
