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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
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- Product number
- R30596 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Collagen IV Antibody / COL4A2 / Canstatin fragment
- Antibody type
- Polyclonal
- Description
- This highly specific Collagen IV antibody is suitable for use in Western blot/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- The lyophilized Collagen IV antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of rat 1) kidney and 2) lung lysate with Canstatin / Collagen IV antibody at 0.5ug/ml. Expected molecular weight of Canstatin: ~24 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human placental tissue lysate with Canstatin / Collagen IV antibody at 0.5ug/ml. Expected molecular weight of Canstatin: 24-30 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F testing of Canstatin / Collagen IV antibody and rat kidney tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse lung tissue with Canstatin / Collagen IV antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast cancer tissue with Canstatin / Collagen IV antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast cancer tissue with Canstatin / Collagen IV antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human placental tissue with Canstatin / Collagen IV antibody at 1ug/ml. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to staining.
