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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- R30596 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Collagen IV Antibody (COL4A2)
- Antibody type
- Polyclonal
- Antigen
- An amino acid sequence from the C-terminus of human Collagen IV COL4A2 (LIRTHISRCQVCMKNL) was used as the immunogen for this Collagen IV antibody (100% homologous in human, mouse and rat).
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- The lyophilized Collagen IV antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of rat 1) kidney and 2) lung lysate with Canstatin / Collagen IV antibody at 0.5ug/ml. Expected molecular weight of Canstatin: ~24 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F testing of Canstatin / Collagen IV antibody and rat kidney tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse lung tissue with Canstatin / Collagen IV antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
