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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA1-25098 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Connexin 43 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: BHK. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GJA1 in mouse brain extract using a GJA1 polyclonal antibody (Product # PA1-25098) at a dilution of 1:8000 and developed with Alk Phos APA Rabbit IgG (gt) and NBT/BCIP substrate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Connexin 43 was performed by loading (1) HEK-293 (2) P19 (3) Rat2 (4) MDCK cells. Proteins were transferred to a membrane and probed with a Connexin 43 Polyclonal Antibody (Product # PA1-25098) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Connexin 43 was performed by loading mouse brain extract. Proteins were transferred to a membrane and probed with a Connexin 43 Polyclonal Antibody (Product # PA1-25098) at a dilution of 1:8000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Connexin 43 using Connexin 43 Polyclonal Antibody (Product # PA1 25098) at 1:400(green) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by methanol: acetone.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Connexin 43 in human heart tissue using Connexin 43 Polyclonal Antibody (Product # PA1-25098) at a dilution of 1:2000.
