C6219

antibody from MilliporeSigma / Merck KGaA

Targeting: GJA1 CX43, GJAL, ODD, ODDD, ODOD, SDTY3

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

C6219

Provider

MilliporeSigma / Merck KGaA

Product name

Anti-Connexin-43 antibody produced in rabbit

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Storage

Store at -20°C

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 43

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows strong cytoplasmic and membranous positivity in myocytes.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.