PPS046

antibody from Novus Biologicals

Targeting: GJA1 CX43, GJAL, ODD, ODDD, ODOD, SDTY3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PPS046

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Connexin 43/GJA1 Antibody

Antibody type

Polyclonal

Description

Connexin 43/GJA1

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Simian, Zebrafish

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 uL

Storage

For long-term storage, = -20degrees C is recommended. Product is stable at = -20degrees C for at least 1 year.

References

Submitted references

Protein kinase Cδ-mediated phosphorylation of Connexin43 gap junction channels causes movement within gap junctions followed by vesicle internalization and protein degradation.

Cone AC, Cavin G, Ambrosi C, Hakozaki H, Wu-Zhang AX, Kunkel MT, Newton AC, Sosinsky GE
The Journal of biological chemistry 2014 Mar 28;289(13):8781-98

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Phospho-Connexin 43/GJA1 (S368) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 43 kDa Connexin 43/GJA1 phosphorylated at S368 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 units of lambda phosphatase ( lambda Phosphatase) for 30 minutes before being exposed to the Anti-Phospho-Connexin 43/GJA1 (S368). The immunolabeling of Connexin43 is completely eliminated by treatment with lambda PPase.