Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AM32558SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti GJA1 / Cx43 (131-142)
- Antibody type
- Monoclonal
- Antigen
- Bovine thyroglobulin coupled peptide corresponding to amino acids 131-142 (EIKKFKYGIEEH-C) of Human Connexin 43 with a C-terminal cysteine added for conjugation.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- A1
- Vial size
- 0.2 ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Immunoblot Analysis: Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Connexin 43 (1/1000 dilution, Lane 2) or without a primary antibody (Lane1).Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Connexin 43 (43kDa). Extra bands also seen in negative control (Lane 1).