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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-36839 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Connexin 43 (Ser367) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 43 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Connexin 43 (Ser367) in Lane 1: SGC7901 whole cell lysate (40 µg), Lane 2: SGC7901 treated with PMA (50 ng/mL,10 minutes) whole cell lysate (40 µg), Lane 3: the Heart tissue lysate of mouse (40 µg), Lane 4: the Testis tissue lysate of rat (40 µg). Samples were incubated with Phospho-Connexin 43 (Ser367) polyclonal antibody (Product # PA5-36839) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Connexin 43 pSer367 using Phospho-Connexin 43 pSer367 polyclonal antibody (Product # PA5-36839) at a dilution of 1:500. Lane 1: Hela cell lysate treated with PMA (100nM, 15min), Lane 2: sp2/0 cell lysate treated with PMA (100nM, 15min), Lane 3: H9C2 cell lysate treated with PMA (100nM, 15min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-Connexin 43 pSer367 in paraffin-embedded human brain tissue using Phospho-Connexin 43 pSer367 polyclonal antibody (Product # PA5-36839).
