PA5-37584

antibody from Invitrogen Antibodies

Targeting: GJA1 CX43, GJAL, ODD, ODDD, ODOD, SDTY3

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-37584

Provider

Invitrogen Antibodies

Product name

Phospho-Connexin 43 (Tyr265) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Connexin 43 antibody detects endogenous levels of Connexin 43 only when phosphorylated at Tyrosine 265.

Concentration

1 mg/mL

References

Submitted references

Vinpocetine Protects Against Cerebral Ischemia-Reperfusion Injury by Targeting Astrocytic Connexin43 via the PI3K/AKT Signaling Pathway.

Zhao M, Hou S, Feng L, Shen P, Nan D, Zhang Y, Wang F, Ma D, Feng J
Frontiers in neuroscience 2020;14:223

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Roles of astrocytic connexin-43, hemichannels, and gap junctions in oxygen-glucose deprivation/reperfusion injury induced neuroinflammation and the possible regulatory mechanisms of salvianolic acid B and carbenoxolone.

Yin X, Feng L, Ma D, Yin P, Wang X, Hou S, Hao Y, Zhang J, Xin M, Feng J
Journal of neuroinflammation 2018 Mar 27;15(1):97

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts from HUVEC cells using Connexin 43 (pTyr265) polyclonal antibody (Product # PA5-37584) (left) or the same antibody preincubated with a blocking peptide (right).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 5 Effects of Vinp on inflammation and apoptosis-related proteins, and the expression of Cx43, p-Cx43, AKT, and p-AKT in the rat cerebral cortex after I/R injury. (A) Representative western blot images and quantification of proteins related to inflammation. (B) Representative western blot images and quantification of proteins related to apoptosis. (C) Representative western blot images of Cx43 and p-Cx43, and quantification of Cx43, p-Cx43, and p-Cx43/Cx43. (D) Representative western blot images of AKT and p-AKT, and quantification of AKT, p-AKT and p-AKT/AKT. Data are shown as the mean +- SEM ( n = 5 in each group). ** P < 0.01, *** P < 0.001 using one-way ANOVA followed by Tukey's post hoc tests. Vinp: Vinpocetine; LY: LY294002; I/R: ischemia/reperfusion.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 6 Effects of Vinp on the expression of Cx43, p-Cx43, AKT, and p-AKT in astrocytes after OGD/R. (A) Representative western blot images of Cx43 and p-Cx43, and quantification of Cx43, p-Cx43, and p-Cx43/Cx43. (B) Representative western blot images of AKT and p-AKT, and quantification of AKT, p-AKT and p-AKT/AKT. Data are shown as the mean +- SEM ( n = 3 in each group). * P < 0.05, ** P < 0.01, *** P < 0.001 using one-way ANOVA followed by Tukey's post hoc tests. Vinp: Vinpocetine; LY: LY294002; OGD/R: oxygen-glucose deprivation/reoxygenation; BKM: BKM120.