138388

antibody from Invitrogen Antibodies

Targeting: GJA1 CX43, GJAL, ODD, ODDD, ODOD, SDTY3

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

138388

Provider

Invitrogen Antibodies

Product name

Connexin 43 Monoclonal Antibody (CX-1B1), Alexa Fluor™ 488

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

CX-1B1

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

Comparative Analysis of Cell-Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models.

Kutova OM, Sencha LM, Pospelov AD, Dobrynina OE, Brilkina AA, Cherkasova EI, Balalaeva IV
Biology 2020 Dec 4;9(12)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart atrium cells stained with Connexin 43 Monoclonal Antibody, Mouse - Alexa Fluor® 488 (Product # 138388), Image courtesy of James I. Nagy, PhD, University of Manitoba, Canada.

Conjugate

Green dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Expression level of analyzed proteins of adherens junctions (E-cadherin), desmosomes (desmoglein-2) and gap junctions (connexin-43) in SKOV-3 and SKOV-3.ip cells cultured in monolayer and 3D in vitro models. ( A , C , E ) The distributions of SKOV-3 cells (left plot) and SKOV-3.ip cells (right plot) according to fluorescence intensity detected after staining with E-cadherin-specific, desmoglein-2-specific and connexin-43-specific antibodies (red--monolayer culture, blue--spheroids, green--collagen hydrogel); ( B , D , F ) Levels of E-cadherin, desmoglein-2 and connexin-43 in monolayer and 3D models denoted as relative fluorescence values, calculated as a ratio of mean fluorescence intensity of cells stained with specific antibodies to mean fluorescence intensity of cells stained with antibodies of isotypic control. mnlr , monolayer; sph , spheroids; clgn , collagen hydrogel. ""*"" indicates significant difference in RF level from monolayer culture (ANOVA, Holm-Sidak's multiple comparisons test, p < 0.05).

Conjugate

Green dye