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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [13]
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- Product number
- R30201 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Cx43 Antibody / Connexin 43
- Antibody type
- Polyclonal
- Description
- This highly specific Cx43 antibody is suitable for use in Western blot/Immunohistochemistry/Immunohistochemistry applications with human (1), mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- The lyophilized Cx43 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Cx43 antibody and rat heart tissue lysate. Predicted molecular weight: 43 kDa
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human placenta, 2) rat brain, 3) rat heart, 4) mouse brain and 5) mouse heart lysate with Cx43 antibody at 0.5ug/ml. Predicted molecular weight ~43 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human placenta, 2) rat brain, 3) rat heart, 4) mouse brain and 5) mouse heart lysate with Cx43 antibody at 0.5ug/ml. Predicted molecular weight ~43 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Cx43 antibody testing of human lung cancer tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Cx43 antibody testing of rat heart tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F testing of rat heart tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human intestinal cancer tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human breast cancer tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of mouse heart tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of rat heart tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Cx43 antibody testing of rat heart tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F testing of rat heart tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human intestinal cancer tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human breast cancer tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of mouse heart tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of rat heart tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
