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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3581 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3581, RRID:AB_1575195
- Product name
- GJA1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of GJA1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Gating and regulation of connexin 43 (Cx43) hemichannels.
Endothelin-1 decreases gap junctional intercellular communication by inducing phosphorylation of connexin 43 in human ovarian carcinoma cells.
Lysosomal and proteasomal degradation play distinct roles in the life cycle of Cx43 in gap junctional intercellular communication-deficient and -competent breast tumor cells.
Contreras JE, Sáez JC, Bukauskas FF, Bennett MV
Proceedings of the National Academy of Sciences of the United States of America 2003 Sep 30;100(20):11388-93
Proceedings of the National Academy of Sciences of the United States of America 2003 Sep 30;100(20):11388-93
Endothelin-1 decreases gap junctional intercellular communication by inducing phosphorylation of connexin 43 in human ovarian carcinoma cells.
Spinella F, Rosanò L, Di Castro V, Nicotra MR, Natali PG, Bagnato A
The Journal of biological chemistry 2003 Oct 17;278(42):41294-301
The Journal of biological chemistry 2003 Oct 17;278(42):41294-301
Lysosomal and proteasomal degradation play distinct roles in the life cycle of Cx43 in gap junctional intercellular communication-deficient and -competent breast tumor cells.
Qin H, Shao Q, Igdoura SA, Alaoui-Jamali MA, Laird DW
The Journal of biological chemistry 2003 Aug 8;278(32):30005-14
The Journal of biological chemistry 2003 Aug 8;278(32):30005-14
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of GJA1 polyclonal antibody (Cat # PAB3581) in HL-60 cell lysate. GJA1 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with GJA1 polyclonal antibody (Cat # PAB3581) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
