Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB5681 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB5681, RRID:AB_1577918
- Product name
- GJA1 (phospho S367) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of GJA1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Alterations of phosphorylation state of connexin 43 during hypoxia and reoxygenation are associated with cardiac function.
Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation.
Connexin43 phosphorylation at S368 is acute during S and G2/M and in response to protein kinase C activation.
Matsushita S, Kurihara H, Watanabe M, Okada T, Sakai T, Amano A
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2006 Mar;54(3):343-53
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2006 Mar;54(3):343-53
Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation.
Bao X, Altenberg GA, Reuss L
American journal of physiology. Cell physiology 2004 Mar;286(3):C647-54
American journal of physiology. Cell physiology 2004 Mar;286(3):C647-54
Connexin43 phosphorylation at S368 is acute during S and G2/M and in response to protein kinase C activation.
Solan JL, Fry MD, TenBroek EM, Lampe PD
Journal of cell science 2003 Jun 1;116(Pt 11):2203-11
Journal of cell science 2003 Jun 1;116(Pt 11):2203-11
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of extract from HeLa (Lane 1, 4), K-562 (Lane 2, 5) and 293 (Lane 3, 6) cells, untreated or treated with PMA (1 uM, 30 min). Using GJA1 (phospho S367) polyclonal antibody (Cat # PAB5681).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GJA1 (phospho S367) polyclonal antibody (Cat # PAB5681).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)