PA5-64641

antibody from Invitrogen Antibodies

Targeting: GJA1 CX43, GJAL, ODD, ODDD, ODOD, SDTY3

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-64641

Provider

Invitrogen Antibodies

Product name

Phospho-Connexin 43 (Ser282) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Phospho-Connexin 43 (Ser282) Polyclonal Antibody detects endogenous levels of Connexin 43 only when phosphorylated at Ser282.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Calmodulin Directly Interacts with the Cx43 Carboxyl-Terminus and Cytoplasmic Loop Containing Three ODDD-Linked Mutants (M147T, R148Q, and T154A) that Retain α-Helical Structure, but Exhibit Loss-of-Function and Cellular Trafficking Defects.

Zheng L, Chenavas S, Kieken F, Trease A, Brownell S, Anbanandam A, Sorgen PL, Spagnol G
Biomolecules 2020 Oct 17;10(10)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Connexin 43 in PMA treated 293 whole cell lysates using a Phospho-Connexin 43 (Ser282) Polyclonal Antibody (Product # PA5-64641).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 9 Determining if the CaM interaction with Cx43CT affects MAPK and Pyk2/Src phosphorylation of the Cx43CT domain. ( a ) Src, ERK1, or ERK2 were used in an in vitro kinase assay performed with purified GST-Cx43CT 236-382 as substrate, in presence or absence of CaM. Phosphorylation was detected by Western blot using Cx43 Y265 or S279/S282 phospho-specific antibodies. ( b ) Quantification of the phosphorylation level from three independent experiments using Figures the iBright Analysis Software and analyzed in GraphPad Prism 8.0 (Student's t -test, *** p < 0.0005, **** p < 0.0001). HeLa Cx43 cells were treated with ionomycin and Ca 2+ , or PMA, or Ca 2+ /PMA for 30 min. Lysates were then subjected to Triton X-100 extraction. Total lysate ( c ) and insoluble ( d ) fractions were analyzed by Western blot. Antibodies used are labeled on the left of each panel. Quantification of the Cx43 pY265 and pS279/282 phosphorylation levels were obtained from three independent experiments using the iBright Analysis Software and analyzed in GraphPad Prism 8.0 (Ordinary one way Anova, * p < 0.05, ** p < 0.01).