A00599
antibody from Boster Biological Technology
Targeting: GJA1
CX43, GJAL, ODD, ODDD, ODOD, SDTY3
Antibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- A00599 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Connexin 43/GJA1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Connexin 43/GJA1 detection. Tested with WB, IHC-P, ICC/IF, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Cellular evidence and spatial distribution of endosomal biosynthesis and autophagy in intestinal immune barrier cells of crucian carp (Carassiuscarassius).
SARS-CoV-2 Structural Proteins Modulated Blood-Testis Barrier-Related Proteins through Autophagy in the Primary Sertoli Cells.
Pinocembrin Inhibits P2X4 Receptor-Mediated Pyroptosis in Hippocampus to Alleviate the Behaviours of Chronic Pain and Depression Comorbidity in Rats.
PGC-1α activator ZLN005 promotes maturation of cardiomyocytes derived from human embryonic stem cells.
Effect of celecoxib on protein expression of FAK and Cx43 in DMBA induced rat tongue carcinoma cells.
Cx32 exerts anti-apoptotic and pro-tumor effects via the epidermal growth factor receptor pathway in hepatocellular carcinoma.
Perineurium-like sheath derived from long-term surviving mesenchymal stem cells confers nerve protection to the injured spinal cord.
Mitochondrial Uncoupling Protein 2 in human cumulus cells is associated with regulating autophagy and apoptosis, maintaining gap junction integrity and progesterone synthesis.
Rehmannia glutinosa oligosaccharide induces differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells.
Ectoderm mesenchymal stem cells promote differentiation and maturation of oligodendrocyte precursor cells.
The compound Chinese medicine "Kang Fu Ling" protects against high power microwave-induced myocardial injury.
Expression of Cx43 and Pax3 proteins in the human placental villi and decidua during early pregnancy.
Recombinant human brain natriuretic peptide therapy combined with bone mesenchymal stem cell transplantation for treating heart failure in rats.
AQP4 KO exacerbating renal dysfunction is mediated by endoplasmic reticulum stress and p66Shc and is attenuated by apocynin and endothelin antagonist CPU0213.
Apocynin and raisanberine alleviate intermittent hypoxia induced abnormal StAR and 3β-HSD and low testosterone by suppressing endoplasmic reticulum stress and activated p66Shc in rat testes.
Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats.
Effects of ischemic preconditioning on ischemia/reperfusion-induced arrhythmias by upregulatation of connexin 43 expression.
Blockade of angiotensin II improves hyperthyroid induced abnormal atrial electrophysiological properties.
Panax notoginseng saponins potentiate osteogenesis of bone marrow stromal cells by modulating gap junction intercellular communication activities.
Differential expression of connexin 43 in human autoimmune thyroid disease.
The separate roles of endothelin receptors participate in remodeling of matrix metalloproteinase and connexin 43 of cardiac fibroblasts in maladaptive response to isoproterenol.
Clinical significance of vascular endothelial growth factor and connexin43 for predicting pancreatic cancer clinicopathologic parameters.
Chemical and physical stimuli induce cardiomyocyte differentiation from stem cells.
Fibrosis of collagen I and remodeling of connexin 43 in atrial myocardium of patients with atrial fibrillation.
Zhu Z, Feng Y, Pan Y, Fan S, Meng X, Wang Y, Liu J, Dai C, Feng J, Peng L, Ma Q, Fang X, Yang P
Fish & shellfish immunology 2024 Jul;150:109605
Fish & shellfish immunology 2024 Jul;150:109605
SARS-CoV-2 Structural Proteins Modulated Blood-Testis Barrier-Related Proteins through Autophagy in the Primary Sertoli Cells.
Kang K, Ma YD, Liu SQ, Huang RW, Chen JJ, An LL, Wu J
Viruses 2023 May 29;15(6)
Viruses 2023 May 29;15(6)
Pinocembrin Inhibits P2X4 Receptor-Mediated Pyroptosis in Hippocampus to Alleviate the Behaviours of Chronic Pain and Depression Comorbidity in Rats.
Yang R, Yang J, Li Z, Su R, Zou L, Li L, Xu X, Li G, Liu S, Liang S, Xu C
Molecular neurobiology 2022 Dec;59(12):7119-7133
Molecular neurobiology 2022 Dec;59(12):7119-7133
PGC-1α activator ZLN005 promotes maturation of cardiomyocytes derived from human embryonic stem cells.
Liu Y, Bai H, Guo F, Thai PN, Luo X, Zhang P, Yang C, Feng X, Zhu D, Guo J, Liang P, Xu Z, Yang H, Lu X
Aging 2020 Apr 28;12(8):7411-7430
Aging 2020 Apr 28;12(8):7411-7430
Effect of celecoxib on protein expression of FAK and Cx43 in DMBA induced rat tongue carcinoma cells.
Shan BZ, Guo B, Li YS, Sun XF
European review for medical and pharmacological sciences 2019 Nov;23(21):9454-9463
European review for medical and pharmacological sciences 2019 Nov;23(21):9454-9463
Cx32 exerts anti-apoptotic and pro-tumor effects via the epidermal growth factor receptor pathway in hepatocellular carcinoma.
Xiang Y, Wang Q, Guo Y, Ge H, Fu Y, Wang X, Tao L
Journal of experimental & clinical cancer research : CR 2019 Apr 4;38(1):145
Journal of experimental & clinical cancer research : CR 2019 Apr 4;38(1):145
Perineurium-like sheath derived from long-term surviving mesenchymal stem cells confers nerve protection to the injured spinal cord.
Ma YH, Zeng X, Qiu XC, Wei QS, Che MT, Ding Y, Liu Z, Wu GH, Sun JH, Pang M, Rong LM, Liu B, Aljuboori Z, Han I, Ling EA, Zeng YS
Biomaterials 2018 Apr;160:37-55
Biomaterials 2018 Apr;160:37-55
Mitochondrial Uncoupling Protein 2 in human cumulus cells is associated with regulating autophagy and apoptosis, maintaining gap junction integrity and progesterone synthesis.
Ge H, Zhang F, Duan P, Zhu N, Zhang J, Ye F, Shan D, Chen H, Lu X, Zhu C, Ge R, Lin Z
Molecular and cellular endocrinology 2017 Mar 5;443:128-137
Molecular and cellular endocrinology 2017 Mar 5;443:128-137
Rehmannia glutinosa oligosaccharide induces differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells.
Wang XH, Du HW, Guo XH, Wang SW, Zhou RB, Li Y, Li ZB, Zhao YS, Zhu QL
Genetics and molecular research : GMR 2016 Oct 17;15(4)
Genetics and molecular research : GMR 2016 Oct 17;15(4)
Ectoderm mesenchymal stem cells promote differentiation and maturation of oligodendrocyte precursor cells.
Zhang Z, Li Z, Deng W, He Q, Wang Q, Shi W, Chen Q, Yang W, Spector M, Gong A, Yu J, Xu X
Biochemical and biophysical research communications 2016 Nov 25;480(4):727-733
Biochemical and biophysical research communications 2016 Nov 25;480(4):727-733
The compound Chinese medicine "Kang Fu Ling" protects against high power microwave-induced myocardial injury.
Zhang X, Gao Y, Dong J, Wang S, Yao B, Zhang J, Hu S, Xu X, Zuo H, Wang L, Zhou H, Zhao L, Peng R
PloS one 2014;9(7):e101532
PloS one 2014;9(7):e101532
Expression of Cx43 and Pax3 proteins in the human placental villi and decidua during early pregnancy.
Zhang J, Zhang L, Liu X, Zhou F
Bio-medical materials and engineering 2014;24(6):3841-7
Bio-medical materials and engineering 2014;24(6):3841-7
Recombinant human brain natriuretic peptide therapy combined with bone mesenchymal stem cell transplantation for treating heart failure in rats.
Zhang J, Zhang L, Wu Q, Liu H, Huang L
Molecular medicine reports 2013 Feb;7(2):628-32
Molecular medicine reports 2013 Feb;7(2):628-32
AQP4 KO exacerbating renal dysfunction is mediated by endoplasmic reticulum stress and p66Shc and is attenuated by apocynin and endothelin antagonist CPU0213.
Cheng YS, Dai DZ, Dai Y
European journal of pharmacology 2013 Dec 5;721(1-3):249-58
European journal of pharmacology 2013 Dec 5;721(1-3):249-58
Apocynin and raisanberine alleviate intermittent hypoxia induced abnormal StAR and 3β-HSD and low testosterone by suppressing endoplasmic reticulum stress and activated p66Shc in rat testes.
Zhang GL, Dai DZ, Zhang C, Dai Y
Reproductive toxicology (Elmsford, N.Y.) 2013 Apr;36:60-70
Reproductive toxicology (Elmsford, N.Y.) 2013 Apr;36:60-70
Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats.
Xing Y, Lv A, Wang L, Yan X, Zhao W, Cao F
Journal of biomedical science 2012 Jan 12;19(1):6
Journal of biomedical science 2012 Jan 12;19(1):6
Effects of ischemic preconditioning on ischemia/reperfusion-induced arrhythmias by upregulatation of connexin 43 expression.
Chen Z, Luo H, Zhuang M, Cai L, Su C, Lei Y, Zou J
Journal of cardiothoracic surgery 2011 Jun 2;6:80
Journal of cardiothoracic surgery 2011 Jun 2;6:80
Blockade of angiotensin II improves hyperthyroid induced abnormal atrial electrophysiological properties.
Xiao P, Gao C, Fan J, Du H, Long Y, Yin Y
Regulatory peptides 2011 Aug 8;169(1-3):31-8
Regulatory peptides 2011 Aug 8;169(1-3):31-8
Panax notoginseng saponins potentiate osteogenesis of bone marrow stromal cells by modulating gap junction intercellular communication activities.
Li XD, Chang B, Chen B, Liu ZY, Liu DX, Wang JS, Hou GQ, Huang DY, Du SX
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2010;26(6):1081-92
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2010;26(6):1081-92
Differential expression of connexin 43 in human autoimmune thyroid disease.
Jiang XY, Feng XH, Li GY, Zhao Q, Yin HQ
Acta histochemica 2010 May;112(3):278-83
Acta histochemica 2010 May;112(3):278-83
The separate roles of endothelin receptors participate in remodeling of matrix metalloproteinase and connexin 43 of cardiac fibroblasts in maladaptive response to isoproterenol.
Peng HJ, Dai DZ, Ji H, Dai Y
European journal of pharmacology 2010 May 25;634(1-3):101-6
European journal of pharmacology 2010 May 25;634(1-3):101-6
Clinical significance of vascular endothelial growth factor and connexin43 for predicting pancreatic cancer clinicopathologic parameters.
Liang QL, Wang BR, Chen GQ, Li GH, Xu YY
Medical oncology (Northwood, London, England) 2010 Dec;27(4):1164-70
Medical oncology (Northwood, London, England) 2010 Dec;27(4):1164-70
Chemical and physical stimuli induce cardiomyocyte differentiation from stem cells.
Ge D, Liu X, Li L, Wu J, Tu Q, Shi Y, Chen H
Biochemical and biophysical research communications 2009 Apr 10;381(3):317-21
Biochemical and biophysical research communications 2009 Apr 10;381(3):317-21
Fibrosis of collagen I and remodeling of connexin 43 in atrial myocardium of patients with atrial fibrillation.
Luo MH, Li YS, Yang KP
Cardiology 2007;107(4):248-53
Cardiology 2007;107(4):248-53
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of GJA1 using anti- GJA1 antibody (A00599). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse testicular tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- GJA1 antigen affinity purified polyclonal antibody (Catalog # A00599) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJA1 at approximately 43KD. The expected band size for GJA1 is at 43KD.
- Additional image