Explore
Validate
LearnPA1026
antibody from Boster Biological Technology
Targeting: GJA1
CX43, GJAL, ODD, ODDD, ODOD, SDTY3
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1026 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Connexin 43/GJA1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Gap junction alpha-1 protein( GJA1) detection. Tested with WB, IHC-P, IF in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration.
Pinocembrin Inhibits P2X4 Receptor-Mediated Pyroptosis in Hippocampus to Alleviate the Behaviours of Chronic Pain and Depression Comorbidity in Rats.
Polystyrene microplastics induce blood-testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats.
Granulocyte colony-stimulating factor attenuates myocardial remodeling and ventricular arrhythmia susceptibility via the JAK2-STAT3 pathway in a rabbit model of coronary microembolization.
Involvement of sphingosine-1-phosphate receptors 2/3 in IR-induced sudden cardiac death.
Perineurium-like sheath derived from long-term surviving mesenchymal stem cells confers nerve protection to the injured spinal cord.
Effect of Novel Gasotransmitter hydrogen sulfide on renal fibrosis and connexins expression in diabetic rats.
Rehmannia glutinosa oligosaccharide induces differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells.
Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43.
Effects of ischemic preconditioning on ischemia/reperfusion-induced arrhythmias by upregulatation of connexin 43 expression.
Blockade of angiotensin II improves hyperthyroid induced abnormal atrial electrophysiological properties.
Panax notoginseng saponins potentiate osteogenesis of bone marrow stromal cells by modulating gap junction intercellular communication activities.
Tan J, Zhuo Z, Wang X, Zhang Y, Qian Y, Lin F
International journal of molecular medicine 2023 Nov;52(5)
International journal of molecular medicine 2023 Nov;52(5)
Pinocembrin Inhibits P2X4 Receptor-Mediated Pyroptosis in Hippocampus to Alleviate the Behaviours of Chronic Pain and Depression Comorbidity in Rats.
Yang R, Yang J, Li Z, Su R, Zou L, Li L, Xu X, Li G, Liu S, Liang S, Xu C
Molecular neurobiology 2022 Dec;59(12):7119-7133
Molecular neurobiology 2022 Dec;59(12):7119-7133
Polystyrene microplastics induce blood-testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats.
Li S, Wang Q, Yu H, Yang L, Sun Y, Xu N, Wang N, Lei Z, Hou J, Jin Y, Zhang H, Li L, Xu F, Zhang L
Environmental science and pollution research international 2021 Sep;28(35):47921-47931
Environmental science and pollution research international 2021 Sep;28(35):47921-47931
Granulocyte colony-stimulating factor attenuates myocardial remodeling and ventricular arrhythmia susceptibility via the JAK2-STAT3 pathway in a rabbit model of coronary microembolization.
Wang W, Ye S, Zhang L, Jiang Q, Chen J, Chen X, Zhang F, Wu H
BMC cardiovascular disorders 2020 Feb 17;20(1):85
BMC cardiovascular disorders 2020 Feb 17;20(1):85
Involvement of sphingosine-1-phosphate receptors 2/3 in IR-induced sudden cardiac death.
Zhang X, Chen D, Wang J, Liu J, Guo H, Zhang G
Heart and vessels 2019 Jun;34(6):1052-1063
Heart and vessels 2019 Jun;34(6):1052-1063
Perineurium-like sheath derived from long-term surviving mesenchymal stem cells confers nerve protection to the injured spinal cord.
Ma YH, Zeng X, Qiu XC, Wei QS, Che MT, Ding Y, Liu Z, Wu GH, Sun JH, Pang M, Rong LM, Liu B, Aljuboori Z, Han I, Ling EA, Zeng YS
Biomaterials 2018 Apr;160:37-55
Biomaterials 2018 Apr;160:37-55
Effect of Novel Gasotransmitter hydrogen sulfide on renal fibrosis and connexins expression in diabetic rats.
Zeng O, Li F, Li Y, Li L, Xiao T, Chu C, Yang J
Bioengineered 2016 Sep 2;7(5):314-320
Bioengineered 2016 Sep 2;7(5):314-320
Rehmannia glutinosa oligosaccharide induces differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells.
Wang XH, Du HW, Guo XH, Wang SW, Zhou RB, Li Y, Li ZB, Zhao YS, Zhu QL
Genetics and molecular research : GMR 2016 Oct 17;15(4)
Genetics and molecular research : GMR 2016 Oct 17;15(4)
Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43.
Xu M, Hu C, Khan HH, Shi FH, Cong XD, Li Q, Dai Y, Dai DZ
Acta pharmacologica Sinica 2016 Feb;37(2):246-54
Acta pharmacologica Sinica 2016 Feb;37(2):246-54
Effects of ischemic preconditioning on ischemia/reperfusion-induced arrhythmias by upregulatation of connexin 43 expression.
Chen Z, Luo H, Zhuang M, Cai L, Su C, Lei Y, Zou J
Journal of cardiothoracic surgery 2011 Jun 2;6:80
Journal of cardiothoracic surgery 2011 Jun 2;6:80
Blockade of angiotensin II improves hyperthyroid induced abnormal atrial electrophysiological properties.
Xiao P, Gao C, Fan J, Du H, Long Y, Yin Y
Regulatory peptides 2011 Aug 8;169(1-3):31-8
Regulatory peptides 2011 Aug 8;169(1-3):31-8
Panax notoginseng saponins potentiate osteogenesis of bone marrow stromal cells by modulating gap junction intercellular communication activities.
Li XD, Chang B, Chen B, Liu ZY, Liu DX, Wang JS, Hou GQ, Huang DY, Du SX
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2010;26(6):1081-92
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2010;26(6):1081-92
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Connexin 43/GJA1 using anti- Connexin 43/GJA1 antibody (PA1026). Connexin 43/GJA1 was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Connexin 43/GJA1 Antibody (PA1026) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
