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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AB0015-500 - Provider product page
- Provider
- SICGEN
- Product name
- Anti-CX43
- Antibody type
- Polyclonal
- Antigen
- Purified recombinant peptide derived from within residues 230 aa to the C-terminus of rat CX43 produced in E. coli.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Zebrafish
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 µl
- Concentration
- 4 mg/ml
- Storage
- For continuous use, store at 2-8°C for one-two days. For extended storage, store in -20°C freezer. Working dilution samples should be discarded if not used within 12 hours.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cell lysates at 100 µg per lane
- Sample type
- COS-7
- Primary Ab dilution
- 1/1,000
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cells were fixed with 4% PFA
- Sample type
- Primary retinal pigmented epithelium
- Primary Ab dilution
- 1/100
- Conjugate
- Unconjugated
