Antibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 25182-1-AP - Provider product page

- Provider
- Proteintech Group
- Product name
- MYH1 (N-terminal) antibody
- Antibody type
- Polyclonal
- Description
- MYH1 (N-terminal) antibody (Cat. #25182-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, IP, ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references [Characterization of the Effect of Tongue on Palatal Shelf Elevation Patterns in a Mouse Model of Palatogenesis].
miRNA-34a-5p inhibits chicken myoblasts proliferation and differentiation via NOTCH1 inhibition.
Conductive MeCbl/PEDOT:PSS/HA hydrogels with electrical stimulation for enhanced peripheral nerve regeneration.
Proteome analysis reveals paraspinal muscle fiber type changes in patients with degenerative lumbar scoliosis.
GPCPD1 is regulated by METTL3/IGF2BP3 dependent m(6)A modification to affect pig muscle fiber type conversion.
Light-phase time-restricted feeding disrupts the muscle clock and insulin sensitivity yet potentially induces muscle fiber remodeling in mice.
Eicosapentaenoic acid-mediated activation of PGAM2 regulates skeletal muscle growth and development via the PI3K/AKT pathway.
miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene.
Dietary leucine and fish oil cooperatively regulate skeletal myofiber type transformation via the CaMKII signaling pathway of pigs.
Dynamic transcriptome profiling reveals essential roles of the receptor tyrosine kinases (RTK) family in feather development of duck.
PHGDH promotes the proliferation and differentiation of primary chicken myoblasts.
miRNA-10a-5p Targeting the BCL6 Gene Regulates Proliferation, Differentiation and Apoptosis of Chicken Myoblasts.
Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway.
MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells.
MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation.
MicroRNA-7 Targets the KLF4 Gene to Regulate the Proliferation and Differentiation of Chicken Primary Myoblasts.
MicroRNA-214 Inhibits Chicken Myoblasts Proliferation, Promotes Their Differentiation, and Targets the TRMT61A Gene.
Does intrauterine crowding affect the force generating capacity and muscle composition of the piglet front limb?
How does intrauterine crowding affect locomotor performance in newborn pigs? A study of force generating capacity and muscle composition of the hind limb.
Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.
Zhang Y, Yang H, Zhang D, Li D, Wang X
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 2025 Sep 20;56(5):1289-1295
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 2025 Sep 20;56(5):1289-1295
miRNA-34a-5p inhibits chicken myoblasts proliferation and differentiation via NOTCH1 inhibition.
Ling X, Wang Q, Zhang J, Zhang H, Zhang L, Zhuo C, Zhang T, Zhang G
Poultry science 2025 Mar;104(3):104895
Poultry science 2025 Mar;104(3):104895
Conductive MeCbl/PEDOT:PSS/HA hydrogels with electrical stimulation for enhanced peripheral nerve regeneration.
Liu K, Shao J, Han B, Liu J, Yan S, Liu B, Liu Y
Materials today. Bio 2025 Jun;32:101755
Materials today. Bio 2025 Jun;32:101755
Proteome analysis reveals paraspinal muscle fiber type changes in patients with degenerative lumbar scoliosis.
Kiram A, Li J, Liu Q, Ling C, Xu H, Fan C, Hu Z, Zhu Z, Qiu Y, Liu Z
The spine journal : official journal of the North American Spine Society 2025 Jun;25(6):1288-1303
The spine journal : official journal of the North American Spine Society 2025 Jun;25(6):1288-1303
GPCPD1 is regulated by METTL3/IGF2BP3 dependent m(6)A modification to affect pig muscle fiber type conversion.
Yang Y, Tan B, Xiao L, Huang H, Xu W, Su J, Zhao Y, Hong L, Cai G, Li Z, Dai L, Gu T
Gene 2025 Aug 20;962:149578
Gene 2025 Aug 20;962:149578
Light-phase time-restricted feeding disrupts the muscle clock and insulin sensitivity yet potentially induces muscle fiber remodeling in mice.
Ye Z, Huang K, Dai X, Gao D, Gu Y, Qian J, Zhang F, Zhai Q
Heliyon 2024 Sep 30;10(18):e37475
Heliyon 2024 Sep 30;10(18):e37475
Eicosapentaenoic acid-mediated activation of PGAM2 regulates skeletal muscle growth and development via the PI3K/AKT pathway.
Li C, Cao H, Ren Y, Jia J, Yang G, Jin J, Shi X
International journal of biological macromolecules 2024 May;268(Pt 2):131547
International journal of biological macromolecules 2024 May;268(Pt 2):131547
miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene.
Zhou KZ, Wu PF, Ling XZ, Zhang J, Wang QF, Zhang XC, Xue Q, Zhang T, Han W, Zhang GX
Poultry science 2024 Jan;103(1):103231
Poultry science 2024 Jan;103(1):103231
Dietary leucine and fish oil cooperatively regulate skeletal myofiber type transformation via the CaMKII signaling pathway of pigs.
Gong S, Yin Y, Han M, Guo L, Duan Y, Guo Q, Yin J, Li F
Food & function 2023 Jan 3;14(1):133-147
Food & function 2023 Jan 3;14(1):133-147
Dynamic transcriptome profiling reveals essential roles of the receptor tyrosine kinases (RTK) family in feather development of duck.
Liu H, Xu Q, Xi Y, Ma S, Wang J, Bai L, Han C, He H, Li L
British poultry science 2022 Oct;63(5):605-612
British poultry science 2022 Oct;63(5):605-612
PHGDH promotes the proliferation and differentiation of primary chicken myoblasts.
Chen L, Wu YL, Ding H, Xie KZ, Zhang T, Zhang GX, Wang JY
British poultry science 2022 Oct;63(5):581-589
British poultry science 2022 Oct;63(5):581-589
miRNA-10a-5p Targeting the BCL6 Gene Regulates Proliferation, Differentiation and Apoptosis of Chicken Myoblasts.
Zhang G, Zhang X, Zhou K, Ling X, Zhang J, Wu P, Zhang T, Xie K, Dai G
International journal of molecular sciences 2022 Aug 23;23(17)
International journal of molecular sciences 2022 Aug 23;23(17)
Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway.
Li P, Zhang S, Song H, Traore SS, Li J, Raubenheimer D, Cui Z, Kou G
Journal of agricultural and food chemistry 2021 Oct 13;69(40):11890-11899
Journal of agricultural and food chemistry 2021 Oct 13;69(40):11890-11899
MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells.
Iwasaki H, Ichihara Y, Morino K, Lemecha M, Sugawara L, Sawano T, Miake J, Sakurai H, Nishi E, Maegawa H, Imamura T
Scientific reports 2021 Jan 13;11(1):1161
Scientific reports 2021 Jan 13;11(1):1161
MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation.
Zhang G, He M, Wu P, Zhang X, Zhou K, Li T, Zhang T, Xie K, Dai G, Wang J
Cells 2021 Feb 17;10(2)
Cells 2021 Feb 17;10(2)
MicroRNA-7 Targets the KLF4 Gene to Regulate the Proliferation and Differentiation of Chicken Primary Myoblasts.
Zhang G, Chen F, Wu P, Li T, He M, Yin X, Shi H, Duan Y, Zhang T, Wang J, Xie K, Dai G
Frontiers in genetics 2020;11:842
Frontiers in genetics 2020;11:842
MicroRNA-214 Inhibits Chicken Myoblasts Proliferation, Promotes Their Differentiation, and Targets the TRMT61A Gene.
Duan Y, Wu Y, Yin X, Li T, Chen F, Wu P, Zhang S, Wang J, Zhang G
Genes 2020 Nov 25;11(12)
Genes 2020 Nov 25;11(12)
Does intrauterine crowding affect the force generating capacity and muscle composition of the piglet front limb?
Vanden Hole C, Van Ginneken C, Prims S, Ayuso M, Van Cruchten S, Aerts P
PloS one 2019;14(10):e0223851
PloS one 2019;14(10):e0223851
How does intrauterine crowding affect locomotor performance in newborn pigs? A study of force generating capacity and muscle composition of the hind limb.
Vanden Hole C, Cleuren S, Van Ginneken C, Prims S, Ayuso M, Van Cruchten S, Aerts P
PloS one 2018;13(12):e0209233
PloS one 2018;13(12):e0209233
Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.
Zhang L, Gong H, Sun Q, Zhao R, Jia Y
Journal of agricultural and food chemistry 2018 Jan 17;66(2):540-550
Journal of agricultural and food chemistry 2018 Jan 17;66(2):540-550
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 25182-1-AP( MYH1 Antibody) at dilution of 1:1000
- Validation comment
- WB
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- IP Result of anti-MYH1 (IP:25182-1-AP, 5ug; Detection:25182-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.
- Validation comment
- IP
Supportive validation
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 25182-1-AP( MYH1 Antibody) at dilution of 1:50 (under 10x lens)
- Validation comment
- IHC
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 25182-1-AP( MYH1 Antibody) at dilution of 1:50 (under 40x lens)
- Validation comment
- IHC