Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C209179 - Provider product page
- Provider
- LSBio
- Product name
- Myosin VI / MYO6 Antibody LS-C209179
- Antibody type
- Polyclonal
- Description
- Delipidated and defibrinated
- Reactivity
- Human, Porcine
- Host
- Rabbit
- Storage
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of Rabbit anti-Myosin-6 antibody. Lane 1: SF9 cell lysate of truncated smooth myosin. Lane 2: Jurkat lysate. Lane 3: LnCap lysate. Lane 4: Recombinant myosin VI Load: 35 ug per lane for cell lysate. 50 ng of recombinant protein. Primary antibody: Myosin 6 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 150 kDa for Myo6.