Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AF5276 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Tryptophan Hydroxylase 1/TPH-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Tryptophan Hydroxylase 1/TPH-1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Tryptophan Hydroxylase 1/TPH-1 by Western Blot. Western blot shows lysates of AGS human gastric adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Tryptophan Hydroxylase 1/TPH-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5276) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Tryptophan Hydroxylase 1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Tryptophan Hydroxylase 1/TPH-1 in Human Brain. Tryptophan Hydroxylase 1/TPH-1 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human Tryptophan Hydroxylase 1/TPH-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5276) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Tryptophan Hydroxylase 1/TPH-1 in Rat Brain. Tryptophan Hydroxylase 1/TPH-1 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Human Tryptophan Hydroxylase 1/TPH-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5276) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.