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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- LS-C761130 - Provider product page
- Provider
- LSBio
- Product name
- TPH1 / Tryptophan Hydroxylase Antibody (phospho-Ser58) LS-C761130
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Rabbit
- Host
- Rabbit
- Storage
- Upon delivery, aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of TPH1 (pS58) expression in HEK293T (A), THP1 (B), MCF7 (C), SGC7901 (D) whole cell lysates.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TPH1 (pS58) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
