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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Product number
- MA5-29618 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TPH1 Recombinant Rabbit Monoclonal Antibody (145)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human Tryptophan Hydroxylase 1/TPH1.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 145
- Vial size
- 100 µL
- Concentration
- 5.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- TPH1 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg THP-1 Whole Cell Lysate 0.5 µL with TPH1 Recombinant Rabbit Monoclonal Antibody (145) (Product # MA5-29618) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: TPH1 Recombinant Rabbit Monoclonal Antibody (145), at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 60 kDa.
