Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
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- Product number
- MA5-29618 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TPH1 Recombinant Rabbit Monoclonal Antibody (145)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Antibody clone number
- 145
- Concentration
- 5.0 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tryptophan Hydroxylase in Lane A: THP1 Whole Cell Lysate (30 µg). Samples were probed using a Tryptophan Hydroxylase Monoclonal Antibody (Product # MA5-29618) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:55 kDa. Observed band size:55 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TPH1 Recombinant Rabbit Monoclonal Antibody (145) (Product # MA5-29618) at 1:500 dilution. Lane A: THP1 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TPH1 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg THP-1 Whole Cell Lysate 0.5 µL with TPH1 Recombinant Rabbit Monoclonal Antibody (145) (Product # MA5-29618) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: TPH1 Recombinant Rabbit Monoclonal Antibody (145), at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 60 kDa.