PA1-777

antibody from Invitrogen Antibodies

Targeting: TPH1 TPH, TPRH

Western blot

Immunoprecipitation

Antibody data

Submit

Validation data

Product number: PA1-777 - Provider product page
Provider: Invitrogen Antibodies
Product name: TPH1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA1-777 detects TPH1 in human, mouse, rat, rabbit and non-human primate samples. PA1-777 has been successfully used in ICC/IF, IP, and Western blot procedures. By Western blot PA1-777 detects a 51 kDa protein representing TPH1. The PA1-777 immunogen is a synthetic peptide corresponding to the C-terminal region of TPH1.
Reactivity: Human, Mouse, Rat, Rabbit
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: 4° C

TPH1 protein structure - PA1-777 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of TPH1 (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TPH1 polyclonal antibody (Product # PA1-777) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of TPH1 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TPH1 polyclonal antibody (Product # PA1-777) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of TPH1 (green) showing staining in the cytoplasm of HUVEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TPH1 polyclonal antibody (Product # PA1-777) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Expression and localization of TPH1/2 in rat tissues The expression and localization of TPH1 (A) and TPH2 (B) were analyzed by immunohistochemistry in the rat brain, liver, and kidney of the healthy, depressive model, and treated groups. The images are representative of six independent experiments.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Protein expression of key serotonin synthesis enzymes is upregulated following HSV-1 Infection. (A) Western blot analysis of DDC, TPH-1, and TPH-2 protein expression between HSV-1-infected and mock-infected A549 cell lysates at 24 and 48 hpi. (B) Quantification from A549 cell western blots of relative expression of DDC, TPH-1, and TPH-2 band intensity normalized to beta-actin. (C) Western blot analysis of TPH-2 protein expression between HSV-1-infected and mock-infected HCEC at 24 hpi. (D) Quantification from HCEC western blots of relative expression of TPH-2 band intensity normalized to beta-actin expressed in HCEC cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Herpes simplex virus upregulation of TPH-1 and TPH-2 expression is associated with a corresponding increase in serotonin production. Cells were mock or HSV-1 infected and at 24 h post infection, expression of TPH-2 (top and middle series of panels, alphaTPH2; red), TPH-1, (bottom series of panels, alphaTPH1; red), serotonin (middle and bottom series of panels, alphaSerotonin; green), or HSV-viral proteins (top series of panels, alphaHSV; green) was visualized by confocal immunofluorescent analysis. Cell nuclei were counterstained with DRAQ5, pseudocolored blue and channels merged.