PA1-4645

antibody from Invitrogen Antibodies

Targeting: TPH1 TPH, TPRH

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-4645

Provider

Invitrogen Antibodies

Product name

Phospho-TPH1 (Ser260) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine, chicken, mouse and zebrafish based on 100% sequence homology. This antibody is specific for the ~55 kDa Tryptophan Hydroxylase protein phosphorylated at Ser260 in Western blots of human dorsal Raphe nucleus.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Activation and stabilization of human tryptophan hydroxylase 2 by phosphorylation and 14-3-3 binding.

Winge I, McKinney JA, Ying M, D'Santos CS, Kleppe R, Knappskog PM, Haavik J
The Biochemical journal 2008 Feb 15;410(1):195-204

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of rat brain stem lysate showing specific immunolabeling of the ~55 kDa TPH phosphorylated at Ser260 (Lane 1).The labeling by Anti-Ser260 PH is specifically blocked by the Ser260 phosphopeptide (Phos-pep) used as antigen. The corresponding non-phosphopeptide (Lane 2) did not block the immunolabeling.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of TPH1 in rat brainstem lysate showing specific immunolabeling of a band at ~55 kDa corresponding to Phospho-TPH1 (Ser260) polyclonal antibody (Product # PA1-4645 in lane one (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by pre-adsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (NP), shown in the third lane.