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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-19749 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Tryptophan Hydroxylase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For Western Blot, this antibody has non-specific bands at 42 kDa. This antibody is predicted to react with rabbit, chicken, hamster, chimpanzee, gorilla and orangutan based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.7 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Human Brain Tissue Lysate using Product # PA5-19749, Tryptophan Hydroxylase primary antibody at a dilution of 1 µg/mL (lane 1). Staining of Mouse Brain Tissue Lysate at a dilution of 1 µg/mL (Lane 2). Staining of Serotoninergic nucleus Rat Tissue Lysate at a dilution of 1 µg/mL (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
