TA503130

antibody from OriGene

Targeting: TUBB4A beta-5, DYT4, TUBB4

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503130

Provider

OriGene

Proper citation

OriGene Cat#TA503130, RRID:AB_11140509

Product name

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI6E3 (formerly 6E3)

Antibody type

Monoclonal

Description

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI6E3 (formerly 6E3)

Host

Mouse

Conjugate

Unconjugated

Epitope

TUBB4A

Isotype

IgG

Antibody clone number

OTI6E3

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB4 (RC203945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB4.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-TUBB4 monoclonal antibody at 1:200 dilution.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from a mouse cell line and a mouse tissues by using anti-TUBB4 monoclonal antibody.(1:200)

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-TUBB4 antibody(TA503130),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC