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Western blot
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Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [2]
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Validation data
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- Product number
- LS-C679924 - Provider product page
- Provider
- LSBio
- Product name
- TUBB4 / Tubulin Beta 4 Antibody LS-C679924
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot All Lanes :TUBB4A antibody at 2 ug/ml Lane 1 : 293T whole cell lysate Lane 2 : Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 50 kDa Observed band size: 50,58 kDa
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot All Lanes :TUBB4A antibody at 2 ug/ml Lane 1 : 293T whole cell lysate Lane 2 : Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 50 kDa Observed band size: 50,58 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating TUBB4A in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of product in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/2000) Lane 2: product (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating TUBB4A in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of product in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/2000) Lane 2: product (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
