Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF2458 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse RGM-A Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse RGM-A in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q6PCX7
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Repulsive Guidance Molecule a (RGMa) Induces Neuropathological and Behavioral Changes That Closely Resemble Parkinson's Disease.
Korecka JA, Moloney EB, Eggers R, Hobo B, Scheffer S, Ras-Verloop N, Pasterkamp RJ, Swaab DF, Smit AB, van Kesteren RE, Bossers K, Verhaagen J
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Sep 27;37(39):9361-9379
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Sep 27;37(39):9361-9379
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- RGM-A in Mouse Embryo. RGM-A was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c) using Goat Anti-Mouse RGM-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2458) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to retina and developing neurons in cross-section of the developing eye. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.