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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-33556 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33556, RRID:AB_2242457
- Product name
- Rabbit Polyclonal Aldo-keto Reductase 1C3/AKR1C3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-33556] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 C: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-33556] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-33556] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.
