PA5-118237

antibody from Invitrogen Antibodies

Targeting: RBM17 MGC14439, SPF45

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118237

Provider

Invitrogen Antibodies

Product name

RBM17 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RBM17 Immunoprecipitation using: Lane A: 0.5 mg Raji Whole Cell Lysate 4 µL with RBM17 Polyclonal Antibody (Product # PA5-118237) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: RBM17 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of RBM17 in Lane A: 0.5 mg Raji Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118237) with a dilution of 1:100 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 45 kDa , Observed band size : 45 kDa .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) of RBM17 in human brain. Samples were incubated with polyclonal antibody (Product # PA5-118237) with a dilution of 1:100 .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RBM17 Immunoprecipitation using: Lane A: 0.5 mg Raji Whole Cell Lysate 4 µL with RBM17 Polyclonal Antibody (Product # PA5-118237) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: RBM17 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa.