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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-63558 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM106B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GKSLSHLPLH SSKEDAYDGV TSENMRNGLV NSEVHNEDGR NGDVSQFPYV EF Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 87%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of TMEM106B was performed by loading 15 µg of HAP1 WT (lane 1) and TMEM106B KO (lane 2) cell lysates in RIPA buffer onto a 4-15% gradient polyacrylamide gel. Proteins on the blot were visualized with Ponceau staining (below immunoblot). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. TMEM106B was detected at approximately 31 kDa using TMEM106B Polyclonal Antibody (Product # PA5-63558) at a dilution of 1:200 in 5% BSA in TBST overnight at 4 degree celsius. The blot was probed with Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Product # 65-6120) diluted to 0.2 µg/mL in TBST with 5% milk for 1 hr at room temperature. Chemiluminescent detection was performed using ECL Western Blotting Substrate. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of TMEM106B in human cell line A549 shows positivity in vesicles. Samples were probed using a TMEM106B Polyclonal Antibody (Product # PA5-63558).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of TMEM106B in human cell line A549 using a TMEM106B Polyclonal Antibody (Product # PA5-63558) shows localization to endosomes and lysosomes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of TMEM106B was performed on HAP1 WT cell lysate. Antibody-bead conjugate was prepared by adding 2 µg of TMEM106B Polyclonal Antibody (Product # PA5-63558) with 30 µL of Dynabeads™ Protein A (Product # 10002D) and rocked for ~2 hour at 4 degree celcius. One mg of protein was incubated with the antibody-bead conjugate for ~2 hours at 4 degree celcius. Following centrifugation and multiple washes, 2% starting material (SM), 2% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a different antibody. Ponceau stained transfer of blot is shown (below immunoblot). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
