PA5-63558

antibody from Invitrogen Antibodies

Targeting: TMEM106B FLJ11273, MGC33727

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-63558

Provider

Invitrogen Antibodies

Product name

TMEM106B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: GKSLSHLPLH SSKEDAYDGV TSENMRNGLV NSEVHNEDGR NGDVSQFPYV EF Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 87%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of TMEM106B in human cell line A549 shows positivity in vesicles. Samples were probed using a TMEM106B Polyclonal Antibody (Product # PA5-63558).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of TMEM106B in human cell line A549 using a TMEM106B Polyclonal Antibody (Product # PA5-63558) shows localization to endosomes and lysosomes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of TMEM106B in human cell line A549 using a TMEM106B Polyclonal Antibody (Product # PA5-63558) shows localization to endosomes and lysosomes.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of TMEM106B was performed on HAP1 WT cell lysate. Antibody-bead conjugate was prepared by adding 2 µg of TMEM106B Polyclonal Antibody (Product # PA5-63558) with 30 µL of Dynabeads™ Protein A (Product # 10002D) and rocked for ~2 hour at 4 degree celcius. One mg of protein was incubated with the antibody-bead conjugate for ~2 hours at 4 degree celcius. Following centrifugation and multiple washes, 2% starting material (SM), 2% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a different antibody. Ponceau stained transfer of blot is shown (below immunoblot). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of TMEM106B in human cerebral cortex using TMEM106B Polyclonal Antibody (Product # PA5-63558) shows positivity in neuronal cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of TMEM106B in human testis using TMEM106B Polyclonal Antibody (Product # PA5-63558) shows cytoplasmic positivity in cells in seminiferous ducts and in Leydig cells..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of TMEM106B in human skeletal muscle using TMEM106B Polyclonal Antibody (Product # PA5-63558) shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of TMEM106B in human cerebellum using TMEM106B Polyclonal Antibody (Product # PA5-63558) shows positivity in Purkinje cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of TMEM106B was performed on HAP1 WT cell lysate. Antibody-bead conjugate was prepared by adding 2 µg of TMEM106B Polyclonal Antibody (Product # PA5-63558) with 30 µL of Dynabeads™ Protein A (Product # 10002D) and rocked for ~2 hour at 4 degree celcius. One mg of protein was incubated with the antibody-bead conjugate for ~2 hours at 4 degree celcius. Following centrifugation and multiple washes, 2% starting material (SM), 2% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a different antibody. Ponceau stained transfer of blot is shown (below immunoblot). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.