PA5-30379

antibody from Invitrogen Antibodies

Targeting: TUBB4B Beta2, TUBB2C

Antibody data

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Validation data

Product number: PA5-30379 - Provider product page
Provider: Invitrogen Antibodies
Product name: Tubulin beta-2C Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, Jurkat, Raji, NIH-3T3.
Concentration: 1 mg/mL

TUBB4B protein structure - PA5-30379 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30µg lysate) of A549 (Lane 1), COS-7 (Lane 2), HT-29 (Lane 3), PC-12 (Lane 4), Neuro-2a (Lane 5) and tissue extract of Rat Brain (Lane 6). The blot was probed with Rabbit Anti-Tubulin beta-2C Polyclonal Antibody (Product # PA5-30379, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). A 50 kDa band corresponding to Tubulin beta-2C was observed across the cell lines and tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30µg lysate) of A549 (Lane 1), COS-7 (Lane 2), HT-29 (Lane 3), PC-12 (Lane 4), Neuro-2a (Lane 5) and tissue extract of Rat Brain (Lane 6). The blot was probed with Rabbit Anti-Tubulin beta-2C Polyclonal Antibody (Product # PA5-30379, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). A 50 kDa band corresponding to Tubulin beta-2C was observed across the cell lines and tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Tubulin-beta-2C was performed by separating 30 µg of 293T lysates by 10% SDS PAGE. Proteins were transferred to a membrane and probed with a Tubulin beta-2C Polyclonal Antibody (Product # PA5-30379) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Tubulin-beta-2C was performed by separating 30 µg of NIH-3T3 lysates by 10% SDS PAGE. Proteins were transferred to a membrane and probed with a Tubulin beta-2C Polyclonal Antibody (Product # PA5-30379) at a dilution of 1:5000.