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LearnAP7637e
antibody from Abgent
Targeting: FGFR2
BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP7637e - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP7637e, RRID:AB_2103508
- Product name
- FGFR2 Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant Protein
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of FGFR2 (arrow) using rabbit polyclonal FGFR2 Antibody (Cat.#AP7637e). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR2 gene (Lane 2) (Origene Technologies).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of FGFR2 Antibody (Cat.#AP7637e) in NCI-H460 cell line lysates (35ug/lane). SFGFR2 (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of FGFR2 Antibody(Cat#AP7637e) with U251 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human lung carcinoma with FGFR2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- TLR4 Antibody (Center) (Cat. #AP7637e) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
