GTX25476

antibody from GeneTex

Targeting: FGFR2 BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX25476

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25476, RRID:AB_380552

Product name

FGFR2 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Runx2 is required for the proliferation of osteoblast progenitors and induces proliferation by regulating Fgfr2 and Fgfr3.

Kawane T, Qin X, Jiang Q, Miyazaki T, Komori H, Yoshida CA, Matsuura-Kawata VKDS, Sakane C, Matsuo Y, Nagai K, Maeno T, Date Y, Nishimura R, Komori T
Scientific reports 2018 Sep 10;8(1):13551

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Transcriptomics and Transposon Mutagenesis Identify Multiple Mechanisms of Resistance to the FGFR Inhibitor AZD4547.

Kas SM, de Ruiter JR, Schipper K, Schut E, Bombardelli L, Wientjens E, Drenth AP, de Korte-Grimmerink R, Mahakena S, Phillips C, Smith PD, Klarenbeek S, van de Wetering K, Berns A, Wessels LFA, Jonkers J
Cancer research 2018 Oct 1;78(19):5668-5679

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The FGFR/MEK/ERK/brachyury pathway is critical for chordoma cell growth and survival.

Hu Y, Mintz A, Shah SR, Quinones-Hinojosa A, Hsu W
Carcinogenesis 2014 Jul;35(7):1491-9

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Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models.

French DM, Lin BC, Wang M, Adams C, Shek T, Hötzel K, Bolon B, Ferrando R, Blackmore C, Schroeder K, Rodriguez LA, Hristopoulos M, Venook R, Ashkenazi A, Desnoyers LR
PloS one 2012;7(5):e36713

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FGFR2 Antibody (N-term) (GTX25476) western blot analysis in mouse NIH-3T3 cell line lysates (35μg/lane). This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of FGFR2 Antibody (N-term) (GTX25476) with Hela cell followed by Alexa Fluor 488-conjμgated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary FGFR2 Antibody (N-term) (GTX25476) , which was peroxidase-conjμgated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry.BC = breast carcinoma

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term)(GTX25476). GTX25476 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H. brain section using FGFR2 Antibody (N-term)(GTX25476). GTX25476 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FGFR2 Antibody (N-term) (GTX25476) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.