Explore
Validate
LearnF50620
antibody from NSJ Bioreagents
Targeting: FGFR2
BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- F50620 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- FGFR2 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific FGFR2 antibody is suitable for use in Immunohistochemistry/Immunofluorescence/Flow cytometry/Western blot/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the FGFR2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human NCI-H460 cell lysate with FGFR2 antibody. Predicted molecular weight of multiple isoforms: 80-120 kDa. The observed size may be larger due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of FGFR2 antibody with U251 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of FGFR2 antibody with U251 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human lung carcinoma with FGFR2 antibody
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human lung carcinoma with FGFR2 antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FGFR2 antibody flow cytometric analysis of U251 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FGFR2 antibody flow cytometric analysis of U251 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
