LS-C2722

antibody from LSBio

Targeting: NEFM NEF3, NF-M, NFM

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C2722

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C2722, RRID:AB_2150068

Product name

NEFM / NF-M Antibody (C-Terminus) LS-C2722

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation

Reactivity

Human, Mouse, Rat, Chicken/Avian, Feline

Host

Chicken/Avian

Storage

Short term: 4°C; Long term: -70°C.

Blocking/Neutralizing

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

160kDa Neurofilament Medium Antibody - Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with NEFM / NF-M Antibody Neurofilament - Medium (NF-M) Antibody, which reveals a strong NF-M band at ~150kDa. Lanes B and C are homogenates of SH-SY5Y cells which were treated with maitotoxin to activate caspase family enzymes (ref. 2). Now a ~100kDa band is seen in addition o the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.