LS-C204562

antibody from LSBio

Targeting: NEFM NEF3, NF-M, NFM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C204562

Provider

LSBio

Product name

NEFM / NF-M Antibody LS-C204562

Antibody type

Polyclonal

Description

Purified

Reactivity

Human

Host

Chicken/Avian

Isotype

IgY

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with NEFM / NF-M antibody, which reveals a strong NF-M band at ~150kDa. Lanes B and C are homogenates of SH-SY5Y cells which were treated with maitotoxin to activate caspase family enzymes (ref. 2). Now a ~100kDa band is seen in addition o the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

View of mixed neuron/glial cultures stained with NEFM / NF-M antibody (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

View of mixed neuron/glial cultures stained with NEFM / NF-M antibody (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

View of mixed neuron/glial cultures stained with NEFM / NF-M antibody (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.