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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN1504239 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Neurofilament Triplet M Protein (NEFM) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Ascites
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 'RMO55
- Vial size
- 0.2 mL
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Immunoblot Analysis: Lambda phosphatase treated (lane 1) and untreated (lane 2) Rat brain microsomal preparations were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-Neurofilament, Medium chain, clone RMO55 (1/5000 dilution). Proteins were visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-Neurofilament, Medium chain (~150kDa).
