NB300-133

antibody from Novus Biologicals

Targeting: NEFM NEF3, NF-M, NFM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-133

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-133, RRID:AB_10003524

Product name

Rabbit Polyclonal NF-M Antibody

Antibody type

Polyclonal

Description

Unpurified. Specifically recognizes the evolutionarily conserved extreme C-terminal region of Neurofilament Medium (~145-170kDa).

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Acid Ceramidase Deficiency in Mice Results in a Broad Range of Central Nervous System Abnormalities.

Sikora J, Dworski S, Jones EE, Kamani MA, Micsenyi MC, Sawada T, Le Faouder P, Bertrand-Michel J, Dupuy A, Dunn CK, Xuan ICY, Casas J, Fabrias G, Hampson DR, Levade T, Drake RR, Medin JA, Walkley SU
The American journal of pathology 2017 Apr;187(4):864-883

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Effect of entacapone on colon motility and ion transport in a rat model of Parkinson's disease.

Li LS, Liu CZ, Xu JD, Zheng LF, Feng XY, Zhang Y, Zhu JX
World journal of gastroenterology 2015 Mar 28;21(12):3509-18

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Oligodendrogenesis and myelinogenesis during postnatal development effect of glatiramer acetate.

From R, Eilam R, Bar-Lev DD, Levin-Zaidman S, Tsoory M, LoPresti P, Sela M, Arnon R, Aharoni R
Glia 2014 Apr;62(4):649-65

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Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance.

Liu HX, Ermilov A, Grachtchouk M, Li L, Gumucio DL, Dlugosz AA, Mistretta CM
Developmental biology 2013 Oct 1;382(1):82-97

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Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering.

Ziv-Polat O, Skaat H, Shahar A, Margel S
International journal of nanomedicine 2012;7:1259-74

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Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex.

Louis ED, Ma K, Babij R, Cortés E, Liem RK, Vonsattel JP, Faust PL
Neuroscience letters 2012 Jun 14;518(1):49-54

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Enhancement of the growth and differentiation of nasal olfactory mucosa cells by the conjugation of growth factors to functional nanoparticles.

Skaat H, Ziv-Polat O, Shahar A, Margel S
Bioconjugate chemistry 2011 Dec 21;22(12):2600-10

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NF-M Antibody [NB300-133] - Analysis of 160kDa Neurofilament Medium expression on whole rat cerebellum homogenate using NB300-133.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NF-M Antibody [NB300-133] - Analysis of neuronal tissue lysates using rabbit pAb to NF-M, NB300-133, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. Strong bands at 145kDa correspond to rodent NF-M molecules, while the NF-M of pig and other larger mammals including humans run at about 160kDa.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: NF-M Antibody [NB300-133] - Staining of cerebral cortex section from Rat with NF-M antibody (red) at 1:5000 dilution. This immunostaining reveals the perikarya of pyramidal neurons and dendrites as well as axons surrounding the neurons. The green channel shows staining with a monoclonal antibody to beta-adrendergic receptor kinase 1.