MAB12268

antibody from Abnova Corporation

Targeting: NEFM NEF3, NF-M, NFM

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB12268

Provider

Abnova Corporation

Product name

NEFM monoclonal antibody, clone 2G12

Antibody type

Monoclonal

Antigen

Recombinant protein corresponding to amino acids 381-443 of human NEFM.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 μg

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of human NEFM recombinant protein (Expected MW is 31.9 kDa) with NEFM monoclonal antibody, clone 2G12 (Cat# MAB12268) at 1:500-1:2000 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [NEFM (AA: 381-443)-hIgGFc transfected HEK293 cells] with NEFM monoclonal antibody, clone 2G12 (Cat# MAB12268) at 1:500-1:2000 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NTERA-2 cell lysate; Lane 2: SK-N-SH cell lysate; Lane 3: PC-12 cell lysate with NEFM monoclonal antibody, clone 2G12 (Cat# MAB12268) at 1:500-1:2000 dilution.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ELISA analysis of NEFM monoclonal antibody, clone 2G12 (Cat# MAB12268) at 1:10000 dilution.

Validation comment

ELISA

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Flow Cytometry analysis of SK-N-SH cells with NEFM monoclonal antibody, clone 2G12 (Cat# MAB12268) at 1:200-1:400 dilution (Green) and negative control (Red).

Validation comment

Flow Cytometry